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点突变抗体 BRAF (V600E) 小鼠单抗/BRaf(V600E) Antibody
点突变抗体 BRAF (V600E) 小鼠单抗/BRaf(V600E) Antibody 货号: 26039 |
| 基因符号: BRaf |
| 描述: Anti-BRaf(V600E) Mouse Monoclonal Antibody |
| 背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers |
| 免疫原: A synthetic peptide from the internal region of BRaf(V600E), human origin |
| 经过测试的应用: ELISA, WB, IHC, IP |
| 推荐稀释比: ELISA: 1: 100-1: 5000 WB: 1: 100-1: 2000 IHC: 1: 100-1: 1000 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2b |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
点突变抗体 BRAF (V600E) 小鼠单抗/BRaf(V600E) Antibody
Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.
Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .
免疫荧光:
Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).
Immunohistochemistry:
B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.
点突变抗体 BRAF (V600E) 小鼠单抗/BRaf(V600E) Antibody
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.Allele specific PCR validated to be Negative for BRAF V600E.
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企业名称
武汉费斯德生物科技有限公司
企业信息已认证
企业类型
有限责任公司(自然人独资)
信用代码
91420103MA7H14773E
成立日期
2022-01-19
注册资本
壹佰万圆整
经营范围
一般项目:细胞技术研发和应用;生物化工产品技术研发;工程和技术研究和试验发展;自然科学研究和试验发展;医学研究和试验发展(除人体干细胞、基因诊断与治疗技术开发和应用);技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广;销售代理;实验分析仪器销售;货物进出口;技术进出口;进出口代理;化工产品销售(不含许可类化工产品)(除许可业务外,可自主依法经营法律法规非禁止或限制的项目)
武汉费斯德生物科技有限公司
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武汉东湖新技术开发区高新大道666号武汉国家生物产业基地项目（生物创新园）B.C.D区研发楼B3-3楼301室
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公司名称： 武汉费斯德生物科技有限公司
公司地址： 武汉东湖新技术开发区高新大道666号武汉国家生物产业基地项目（生物创新园）B.C.D区研发楼B3-3楼301室 联系人： 黎敏 邮编： 430074
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