宏基因组测序

Introduction

Metagenomic sequencing extracts and high-throughput sequencing all microbial genomic DNA from environmental samples, and analyzes all microbial genomic information in the sample environment. Metagenome sequencing interprets the diversity and abundance information of microbial population, analyzes the genome and function of microbial population, explores the relationship between microorganism and environment, microorganism and host, and explores and studies new functional genes.

1. Content of bioinformatics analysis 

1.1 Sequencing quality analysis, statistics and evaluation 

1.2 Database comparison

1.3 Species abundance analysis

1.4 Functional gene annotation

1.5 Difference analysis of gene functional abundance

1.6 Pathway enrichment analysis

1.7 Abundance difference gene and species cluster analysis

1.8 PCA analysis

2. Sample requirements 

2.1 Total amount > 5 µg gDNA

2.2 Concentration > 100 ng/µL 

2.3 260/280 > 1.8