大鼠白介素6(IL-6)ELISA试剂盒技术原理

　　
　　大鼠白介素6(IL-6)ELISA试剂盒技术原理

　　英文名Rat IL-6(Interleukin 6)ELISA Kit

　　别名IL6;BSF2;HGF;HSF;IFNB2;CDF

　　产品描述

　　应用：用于大鼠血清、血浆或其他相关生物液体中白介素6的测定。

　　For the determination of Rat IL-6(Interleukin 6)concentrations in serum,plasma and other related biological fluids.

　　大鼠白介素6(IL-6)ELISA试剂盒技术规格

　　工作原理本试剂盒采用的是双抗体夹心法酶联免疫吸附检测技术（ELISA）。测定样品中大鼠白介素6水平。向预先包被了抗大鼠白介素6抗体的酶标孔中，加入标准品和样本，温育后，加入生物素标记的抗白介素6抗体。再与HRP标记的链霉亲和素结合，形成免疫复合物，再经过温育和洗涤，去除未结合的酶，然后加入显色底物TMB，产生蓝色，并在酸的作用下转化成最终的黄色。最后，在450 nm处测定反应孔样品吸光度（OD）值，样本中的大鼠白介素6浓度与OD值成正比，通过绘制标准曲线计算出样本中大鼠白介素6的浓度。

　　Purpose This assay employs the Sandwich enzyme immunoassay technique.Determine the level of Rat IL-6(Interleukin 6)in the sample.Add standard and sample to the microplate that have been pre-coated with Anti-Rat IL-6(Interleukin 6)antibody.After incubation,add Biotin-conjugated anti-Rat IL-6(Interleukin 6)antibody.It is then combined with HRP-conjugated streptavidin to form an immune complex,then incubated and washed to remove unbound enzyme,and then added to the chromogenic substrate TMB to produce a blue color,and converted to the final yellow under the action of acid.Finally,the absorbance(OD)value was measured at 450 nm.The concentration of Rat IL-6(Interleukin 6)in the sample was proportional to the OD value.The concentration of Rat IL-6(Interleukin 6)in the sample can be calculated by drawing a standard curve.

　　大鼠白介素6(IL-6)ELISA试剂盒古朵生物

　　灵敏度7.5 pg/mL

　　Sensitivity 7.5 pg/mL

　　检测范围12.5-800 pg/mL

　　Examination range 12.5-800 pg/mL

　　特异性可检测样本中的大鼠白介素6，且与其类似物无明显交叉反应。

　　Specificity This kit recognizes Rat IL-6(Interleukin 6)in samples.No significant cross-reactivity or interference between Rat IL-6(Interleukin 6)and analogues was observed.

　　重复性三种不同浓度的样本在同一块板子上分别检测20次，以及在不同的板子上分别检测20次，板内和板间变异系数均小于10%。

　　Repeatability Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards.Within the plate or between the plates,the coefficient of variation is<10%.

　　回收率分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。

　　Recovery A known concentration of the target protein was added to 5 samples which were diluted to different multiples,and a recovery experiment was performed to obtain a recovery range and an average recovery rate.