CTLA-4 Protein, Human, Recombinant (His & hFc Tag)
纯度:> 92 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.2-1μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
蛋白构建:A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
NP No.:NP_005205.2
表达宿主:HEK293 Cells
种属:Human
预测 N 端:Lys 36
分子量:The recombinant human CTLA4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 375 amino acids and has a predicted molecular mass of 41.8 kDa. As a result of glycosylation, the apparent molecular mass of rh CTLA4/Fc monomer is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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