ASAM Protein, Rat, Recombinant (His Tag)
纯度:> 85 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 10E4 cells/well are added to Recombinant Rat ASAM coated plates (12.5 μg/mL with 100 μL/well), 35-70% cells will adhere after 1 hour incubation at 37℃.
蛋白构建:A DNA sequence encoding the rat ASAM (Q8K1G0) (Met1-Met232) was expressed, fused with a polyhistidine tag at the C-terminus.
Uniprot No.:Q8K1G0
表达宿主:HEK293 Cells
种属:Rat
预测 N 端:Thr 18
分子量:The recombinant rat ASAM comprises 226 amino acids and predicts a molecular mass of 25.6 kDa. The apparent molecular mass of the recombinant protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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