PCSK9 Protein, Mouse, Recombinant (His Tag)
纯度:> 95 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:Measured by its binding ability in a functional ELISA . Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR . The EC50 of biotinylated human LDLR is 0.12 μg/ml.
蛋白构建:A DNA sequence encoding the full length of mouse PCSK9 (NP_705793.1) precursor (Met 1-Gln 694) was expressed, with a C-terminal polyhistidine tag.
NP No.:NP_705793.1
表达宿主:HEK293 Cells
种属:Mouse
预测 N 端:Gln 35
分子量:The secreted recombinant mouse PCSK9 consists of 671 amino acids and has a calculated molecular mass of 72.6 kDa. As a result of proteolysis and glycosylation, the recombinant protein migrates as doublet with apparent molecular mass of approximately 19 & 65 kDa, cprresponding to the prodomain and the mature form of PCSK9 respectively in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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