Fibronectin Protein, Human, Recombinant (His Tag)
别称:CIG Protein, Human; ED-B Protein, Human; FINC Protein, Human; FN Protein, Human; FNZ Protein, Human; GFND Protein, Human; GFND2 Protein, Human; LETS Protein, Human; MSF Protein, Human
纯度:> 97 % as determined by SDS-PAGE.
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.
When cells are added to Fibronectin coated plates (2.5 μg/mL and 100 μL/well), approximately 35%-60% cells will adhere specifically after 35 minutes at 37℃.
蛋白构建:A DNA sequence encoding the Fragment 2 (Ser607-Pro1265) of human Fibronectin (NP_997647.1) was expressed with a C-terminal polyhistidine tag.
NP No.:NP_997647.1
表达宿主:HEK293 Cells
种属:Human
预测 N 端:Ser 607
分子量:The secreted recombinant human Fibronectin 1 fragment2 (FN1.2) consists of 670 amino acids and has a calculated molecular mass of 73.2 kDa. As a result of glycosylation, rhFN1.2 migrates as an approximately 94.1 kDa band in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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