Legumain Protein, Mouse, Recombinant (His Tag)
别称:AEP Protein, Mouse; AI746452 Protein, Mouse; AU022324 Protein, Mouse; Prsc1 Protein, Mouse
纯度:> 75 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC).
The specific activity is > 350 pmoles/min/μg.
(Activation description: The enzyme achieves its activity under acidic pH)
蛋白构建:A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.
NP No.:NP_035305.1
表达宿主:HEK293 Cells
种属:Mouse
预测 N 端:Thr 22
分子量:The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.