Cryptococcus gattii

平台编号：Bio-105124

Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout 拉丁名

ATCC MYA-4093 编号

Deposited As Cryptococcus bacillisporus

Strain Designations 菌种别名 A1M R265 [CBS 10514]

Alternate State Filobasidiella bacillispora Kwon-Chung, teleomorph

Application 用途

Multigene phylogeny and phenotypic characterization.

Biomedical Research and Development Material

Biosafety Level 安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Storage Conditions 培养条件

Frozen 冷冻物 : -80°C or colder

Freeze-Dried 冻干物 : 2°C to 8°C

Live Culture: See Propagation Section

Type Strain 模式菌株 no

Antigenic Properties Serotype B

Genotype VGIIa, AFLP6A

Preceptrol no

Genome Sequenced Strain 

Yes

Mating Type alpha

Comments 注释

Genome sequencing strain (Broad Institute, USA)

Cause cryptococcosis

Represents one of the two genotypes causing an outbreak in Vancouver Island

Multigene phylogeny and phenotypic characterization

Designated as type strain of Cryptococcus deuterogattii Hagen & Boekhout by Hagen et al. (2015)

Medium 培养基 ATCC Medium 28: Emmons' modification of Sabouraud's agar

ATCC Medium 200: YM agar or YM broth

ATCC Medium 1245: YEPD

Growth Conditions 生长条件

Temperature 培养温度 : 24°C to 26°C

Atmosphere 需氧情况 : Typical aerobic

Sequenced Data 

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATACTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCAGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC

26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence

ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAATATGTTAGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGAGGCTTTGCTTTTTTTTATGACGATGACTTGTCAAAGTGTGGATGACTCCAGGAGAGAGGTCGGGCTGGTAATTAGTAATATCAGTCAGTCATTTCAGCTGGCGCCATCGATACTTTATAAGTCAAACTTAACCATTTCACTGTGTAGTAAGTAGTAGGCTCTGAATTACTAGAGACACTTGCCAGGTTATCGCAAGTTGGCAGGCAGGCAGGACACACATACTATTGATTTCACGGTCATGGGGGACTTGGCTGCTGGTGCTTGAGTTGCATAATAGAGAGAGTATATGCATGTCGCGGGGGGGGGACTTGGCTAAGATGCGTTATGCGGCAAGCAAGGTAAGTAGTATTTGTATTAAAGTATTTAGTTACTTTTCCAGTCATGGGGGACTTTGATAGTGGTTGTGAGCCGGAATGAAAAATGGGTAAATGTGGTATGGATGGTGAGAGGGGGAGCTGCCAAGTGTATGCAGTGGGTGTGCTTATAGCATAGTCAAGAGGGGATGGGCAATTAAATTTTGGAAAGTTGTTTGACCCGACCTGACGGTGACTCTAATAGGCTGGTCAAGCAAACGTTTAAGTTGAGTCAAGCTCGGTCAAGCAAAGTCTAGAAAAGATTAATGATCGCTAAGAATTACTCCGGTCGCGGGGGGCTTGCAACTTGTCTGGCCCACTGTGGGTCCAGTTT

glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds

TACTTGCATAAATACAATGGTTGTCAAGGTTGGAATCAACGGTTTCGGTACGTGATCTGTCTCTATCAAGCGTTGCTTCATATGTCACCAATGTGGTTTCCGATATTGTGCACCGTCCTTGGGTTACTGGTAGCTAATAGTTATGCGATAGGTCGTATCGGTCGAATTGTTCTCAGGTATGCTTTAAGTCAAGGGGTAAGACAGCTGCGTATCTAACCATCAAATTAGGAACGCCATCGAGCATGGAGACCTTGAGGTTGTTGCTGTCAACGAGTGAGTATTCTCAAAAGTGTTAATCTATTAAATAAATTATTTACTTTTGCAAAGCCCTTTCATTGACTTGGACTACATGGTATAATACTTTCTTCTGTACAATTGCTTCGTTTTGTGCTAACTGTAGCATGCGTAGGTTTACATGTTCAAGTACGACTCCGTAAGTCTCTGATGGCTCTAGCGCTCTATATGGCATTTTAACCTCATTCCCAACAGACACATGGTCGCTTCAAGGGTTCTGTCGAGGTTAAGGACGGTAAGCTCTATATCAACAACAAGGCCATTTCCGTTTTCGGTGAGAAGGACCCCGCCAACATCAAGTGGGGTGACGCTGGTGCCGAGTACATCGTTGAGTCTACCGGTGTCTTCACCACTACCGAAAAGGCCGGTGTCCAT

Name of Depositor 寄存者 L Hoang

Special Collection NSF - Mycology

Chain of Custody 来源国家 ATCC <-- L. Hoang

Isolation 分离基物

Bronchial alveolar lavage, Duncan, Vancouver Island, B.C. Canada.

Cross References 

Nucleotide (GenBank) : AAFP00000000 Cryptococcus gattii VGII R265, whole genome shotgun sequencing project

References 参考文献

Hu G, Kronstad JW. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition. Curr Genet 49: 341-350, 2006. PubMed: 16397763

Fraser James A, et al. Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak. Nature 437: 1360-1364, 2005.

Kidd Sarah E, et al. Comparative gene genealogies indicate that two clonal lineages of Cryptococcus gattii in British Columbia resemble strains from other geographical areas. Eukaryot. Cell 4: 1629-1638, 2005.

Kidd Sarah E, et al. A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc. Natl. Acad. Sci. USA 101: 17258-17263, 2004.

Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334

D'Souza CA, et al. Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts. mBio 2: e00342-10, 2011. PubMed: 21304167

Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988