Leishmania donovani

平台编号：Bio-109103

Leishmania donovani (Laveran and Mesnil) Ross 拉丁名ATCC 50212 编号Deposited As Leishmania donovani donovani

Strain Designations MHOM/IN/80/DD8

Application 用途

Vector borne research

Biosafety Level 安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Bone marrow of 9-year-old Indian male, Bihar, India, 1980

Product Format 提供形式 frozen

Storage Conditions 培养条件Frozen Cultures:

-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:

2-8°C

Live Cultures:

See Protocols section for handling information

Type Strain 模式菌株 no

Comments 

WHO reference strain. Promastigotes.

Selective killing of amastigotes

Medium 培养基 ATCC Medium 807: Brain heart infusion blood agar

ATCC Medium 1011: Diphasic blood agar medium

ATCC Medium 1012: Diphasic blood agar medium

ATCC Medium 431: Trypanosome medium

Growth Conditions 生长条件

Temperature 培养温度 : 25°C

Culture System 需氧情况 : Axenic

Cryopreservation Harvest and Preservation

Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.

Adjust concentration of cells to 2 x 107/mL in fresh medium.

While cells are centrifuging prepare a 10% (v) solution of sterile DMSO in fresh medium (broth).  The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.

Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.

Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

Place the ampules in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  

Store in either the vapor or liquid phase of a nitrogen refrigerator.

To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after thawed.

Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh tube of ATCC medium 807. 

Incubate vertically at 25°C with the cap screwed on tightly.

Maintain as described above.  

Name of Depositor 寄存者 WRAIR

Chain of Custody 来源国家 ATCC <-- WRAIR <-- W. Peters <-- L. Srivistava

Year of Origin 1980

References 参考文献

Xu ZB, et al. The characterization by isoenzyme electrophoresis of Leishmania isolated in the People's Republic of China. Trans. R. Soc. Trop. Med. Hyg. 78: 689-693, 1984. PubMed: 6506159

Muyombwe A, et al. Selective killing of Leishmania amastigotes expressing a thymidine kinase suicide gene. Exp. Parasitol. 85: 35-42, 1997. PubMed: 9024200