Trichomonas vaginalis

平台编号：Bio-109870

 Trichomonas vaginalis Donne 拉丁名 

(ATCC® 30238™) 统一编号

Strain Designations 别名 JH 32A #4

Application

Sexually Transmitted Disease Research

Biosafety Level 生物安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation 用途 Endocervical swab, Johns Hopkins Hosp., Baltimore, MD, 1963

Product Format 提供形式 frozen

Storage Conditions 藏条件 Frozen Cultures:

-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:

2-8°C

Live Cultures:

See Protocols section for handling information

Type Strain 模式菌株 no

Antibiotic Resistance

Metronidazole resistant.

Comments 注释

Metronidazole resistant.

Viability at four temperatures

Specific and common antigens

Virus RNAs

Medium 培养基 ATCC® Medium 2154: LYI Entamoeba medium

ATCC® Medium 361: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 6.0 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use

Growth Conditions 生长条件 Temperature: 35°C

Atmosphere: Anaerobic 

Culture System: Axenic; pH 6.0

Cryopreservation Harvest and Preservation

Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.

Adjust the concentration of cells to 2 x 106  to  2 x 107/mL in fresh medium.

While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.

Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube.

Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium.

Invert several times to dissolve the DMSO.

Allow to warm to room temperature.

Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to  107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.

Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  

The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.

To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.

Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0.

Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154).

Name of Depositor BM Honigberg

Year of Origin 1963

References 参考文献  

Lecke SB, et al. Trichomonas vaginalis: microtubule cytoskeleton distribution using fluorescent taxoid. Exp. Parasitol. 102: 113-116, 2002.

Mattos A, et al. Fine structure and isozymic characterization of trichomonadid protozoa. Parasitol. Res. 83: 290-295, 1997. PubMed: 9089728

Rosset I, et al. Scanning electron microscopy in the investigation of the in vitro hemolytic activity of Trichomonas vaginalis. Parasitol. Res. 88: 356-359, 2002. PubMed: 11999024

Smith RF. Viability of Trichomonas vaginalis in vitro at four temperatures. J. Clin. Microbiol. 18: 834-836, 1983. PubMed: 6605364

Tai JH, et al. The divergence of Trichomonas vaginalis virus RNAs among various isolates of Trichomonas vaginalis. Exp. Parasitol. 76: 278-286, 1993. PubMed: 8500587

Torian BE, et al. Specific and common antigens of Trichomonas vaginalis detected by monoclonal antibodies. Infect. Immun. 43: 270-275, 1984. PubMed: 6360900