Farage

平台编号：Bio-135035

Farage

CRL-2630™

Farage is a B lymphocyte cell line that was isolated in 1990 from a White, adult female patient with  non-Hodgkin's B Cell lymphoma. This cell line was deposited by H Ben-Bassat and can be used in immunology research.

Product category

Human cells

Organism

Homo sapiens, human

Cell type

B lymphocyte

Morphology

lymphoblast

Disease

Lymphoma; Non-Hodgkin's B Cell

Applications

3D cell culture

Immunology

Product format

Frozen

Characteristics

Growth properties

Suspension

Derivation

The Farage cell line was adapted to culture in 1990 from a lymph node biopsy of a patient with diffuse large cell non-Hodgkin's lymphoma (DLCL).

Age

adult

Ethnicity

White

Gender

Female

Karyotype

trisomy of chromosome 11

Metastatic

Lymph node

Antigen expression

CD10 +/-; CD11a + (LFA-1); CD19 +; CD20 +; CD21 +; CD22 +; CD23 +; CD29 (VLA-4) +; CD38 +; CD39 +; CD40 +; CD44 +; CD54 + (ICAM-1); CD58 + (LFA-3); CD23 -; HLA DR +

Comments

The cells do not express surface or cytoplasmic immunoglobulin.

Exposure to IL-4 augmented the concentrations of CD23, CD54, and CD58 but diminished the expression of CD21, CD22, and CD38. Incubation with IL-4 for 6 to 8 days led to increased expression of CD11a, CD39, CD40, and to disappearance of CD21 and CD38.

Exposure of Farage cells to phorbol 12-myristate 13-acetate (PMA) down-regulated CD21 and CD23 expression.

They do not express the terminal deoxynucleotydyl transferase gene (TdT), nor the recombination activating genes RAG-1 and RAG-2, known as markers of the pre-B cell stage. These results show that Farage represents a mature B-cell rather than a pre-B cell.

The cells are positive for Epstein-Barr virus (EBV).

mature B cell

Handling information

Unpacking and storage instructions

Check all containers for leakage or breakage.

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Temperature

37°C

Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

It is recommended that the cryoprotective agent be removed immediately.  Centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes.

Transfer the cell pellet to an appropriate size vessel (see the specific batch information for the culture recommended dilution ratio).  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.

Subculturing procedure

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 - 5 x 105 viable cells/mL. Maintain cell density between 3 x 105 and 3 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Reagents for cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)

Quality control specifications

History

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