Sideroxydans lithotrophicus

平台编号：Bio-136908

Sideroxydans lithotrophicus

700298 ™

Product category

Bacteria

Strain designation

ES-1

Type strain

Yes

Isolation source

Groundwater tile drain; isolated by D. Emerson

Product format

Frozen

General

Preceptrol

No

Handling information

Medium

ATCC Medium 7266: Modified Wolfe's Mineral Medium Gradient Plate Medium

Temperature

25°C

Atmosphere

Microaerophilic: 3-5% O2, 10% CO2

Handling procedure

1. One frozen vial should be used to start no more than three gradient plates.

2. Autoclave the top and bottom layers for 20 minutes at 121°C.  Cool the top layer in an ice bath while allowing the bottom layer to cool slightly.

3. Pipette 8.5 ml of the bottom layer into a standard Petri dish.  Allow to set a minimum of 15 minutes, but no longer than 30 minutes. 

4. While the bottom layer is setting, adjust the pH of the top layer to between 6.0 and 6.4 by sparging with filter-sterilized CO2.

5. Inoculate the top layer with the vial of S. lithotrophicus and pipette 16 ml over the solidified bottom layer.

6. Place the plates in either GasPak jars with BD BBL CampyPak Plus Microaerophilic system envelopes with palladium catalysts or Mitsubishi AnaeroPack system jars with Pack-MicroAero gas generating envelopes adjusted for the volume of the container.

Handling notes

        Growth should be checked by epifluorescent microscopy five days after inoculation.  Syto 13 (Molecular Probes, Eugene, OR) provides good penetration of the Fe-oxides and bright fluorescence.  A 0.25 mM stock solution is prepared in sterile d-H2O that is then diluted 1:5 with the bacterial culture.  Cells are long, helical and vibroid shaped, less than 0.5 um in diameter, and motile during exponential growth.

        Additional volume may be obtained by concentrating cells by centrifugation and using the entire pellet as inoculum for further growth.  For example, three plates may be used as inoculum for 12 plates, and the pellet from 12 plates may be used to inoculate 36 plates.

          Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as

Unidentified bacterium

Depositors

D Emerson

Type of isolate

Environmental

Cross references

GenBank DQ386264 Siderooxidans lithoautotrophicus strain ES-1 16S ribosomal RNA gene, complete sequence

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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