pIEFBPR(同源臂有碱基突变，不影响功能）

pIEFBPR(同源臂有碱基突变，不影响功能）

复制子: ColE1 Ori

质粒分类: 枯草杆菌质粒；枯草整合质粒；枯草分泌质粒

质粒大小: 8965bp

原核抗性: Amp

克隆菌株: DH5a

培养条件: 37度

质粒属性

载体宿主： 枯草杆菌

载体用途： 蛋白表达

基因种属：

基因类型： ORF

原核抗性： Amp

真核抗性：

荧光蛋白：

质粒简介

           pIEFBPR质粒是一个枯草整合型分泌载体，该质粒的参考文献：mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis

           ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue.