大肠杆菌噬菌体MS2

                   大肠杆菌噬菌体MS2 百欧博伟生物

大肠杆菌噬菌体，是指寄生在大肠杆菌内的一种噬菌体，属于细胞病毒的一种。它一旦离开宿主细胞，就无法复制、生长。

基本信息

平台编号：bio-49215

规格：freeze-dried

拉丁属名：Escherichia coli bacteriophage MS2

菌株名称：大肠杆菌噬菌体MS2

外文名：coliphage 

寄生体：大肠杆菌 

属性：细胞病毒 

类别：生物学名词

注意事项：仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用

产品概述

ATCC® Number:15597-B1™

Host Organism: Escherichia coli (Migula) Castellani and Chalmers ATCC15597

Designations: MS2

Depositors: JH Strauss 

History: ATCC<<--JH Strauss<<--A. Clark

Biosafety Level:1

Shipped: freeze-dried 

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: Control Culture [92378] coliphages in water [92490] control strain [89532] [89533] detection of [92385] detection of bacteriophages [92809] testing [92365] [92369]

References : 5425: Strauss H Jr., Sinsheimer RL. Purification and properties of bacteriophage MS2 and of its ribonucleic acid. J. Mol. Biol. 7: 43-54, 1963. PubMed: 13978804 89532: Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1601, 2001 89533: Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1602, 2001 92365: Inactivation of MS2 virus in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 02/03/EPADWCTR. 92369: Protocal for Equipment Verification Testing for Physical Removal of Microbiological and Particulate Contaminants. Washington, DC:Environmental Protection Agency;EPA EPA 05/9205/EPADWCTR. 92378: USEPA Manuual of Method for Virology, Chapeter 16. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (N16), 2001 92385: Water quality--Detection and enumeration of bacteriophages--Part 1: Enumeration of F-specific RNA bacteriophages. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10705-1:1995. 92490: 9224 C: Male-specific coliphage assay using Escherichia coli Famp. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA9224 - Detection of Coliphages;. 92809: Water quality --- Detection and enumeration of bacteriophages --- Part 1: Enumeration of F-specific RNA bacteriophages [BS EN ISO 10705-1:2001]. London, UK:British Standards Institution;British Standard BS 6068-4.11:1996.

菌种简介

大肠杆菌噬菌体，是指寄生在大肠杆菌内的一种噬菌体，属于细胞病毒的一种。噬菌体还有一个特点就是具有专一性,一种噬菌体只在一种细菌中生活,而不能在另一种细菌中生活,人噬菌体是一种大肠杆菌的温和噬菌体,它是遗传工程中极好的载体分子,就是担当把动物的基因搬运进大肠杆菌细胞的搬运工角色。

菌种繁殖特点

繁殖过程可以分为吸附、侵入、复制、合成与释放4步。

（1）吸附。噬菌体以它的尾部末端高度特异性地吸附到敏感细胞表面上某一特定的位点，如细胞壁、鞭毛或纤毛。

（2）侵入。将自身的遗传物质（DNA）注入宿主细胞内。

（3）复制与合成。指噬菌体DNA和蛋白质外壳的复制。噬菌体DNA进入宿主细胞后，立即引起宿主的代谢改变，宿主细胞内的核酸不能按自身的遗传特性复制和合成蛋白质，而由噬菌体核酸所携带的遗传信息控制，借用宿主细胞的合成机构及酶等复制核酸，进而合成噬菌体的蛋白质。核酸和蛋白质装配合成新的噬菌体。

（4）释放。噬菌体粒子成熟后，噬菌体的水解酶水解宿主细胞而使宿主细胞裂解，噬菌体被释放出来。

大肠杆菌噬菌体并不会引起大肠杆菌所导致的一切病症。