ATCC 30184 阴道毛滴虫 百欧博伟生物

               ATCC 30184 阴道毛滴虫 百欧博伟生物

阴道毛滴虫（Trichomonas vaginalis, Donne 1837）是寄生在人体阴道和泌尿道的鞭毛虫，主要引起滴虫性阴道炎和尿道炎，是以性传播为主的一种传染病。

基本信息

平台编号：bio-48621

规格：frozen

拉丁属名：Trichomonas vaginalis Donne

中文名：阴道毛滴虫

释  义： 寄生在人体阴道和泌尿道的鞭毛虫 

详情介绍

Trichomonas vaginalis Donne 拉丁名

ATCC 30184 编号

Strain Designations 菌株别名 123414

本菌形态：阴道毛滴虫的生活史仅有滋养体阶段而无包囊阶段。活体呈无色透明，有折光性，体态多变，活动力强。固定染色后呈梨形，体长7～23μm，前端有一个泡状核，核上缘有5颗排列成环状的基体，由此发出5根鞭毛：4根前鞭毛，1根后鞭毛。1根轴柱，纤细透明，纵贯虫体，自后端伸出体外。体外侧前1/2处，有一波动膜，其外缘与向后延伸的后鞭毛相连。虫体借助鞭毛摆动前进，以波动膜的波动作旋转式运动。胞质内有深染的颗粒，为该虫特有的氢化酶体（hydrogenosome）。

Application 用途

Sexually Transmitted Disease Research

Biosafety Level 安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Vaginal swab; Nashville, TN; 1964

Product Format 提供形式 frozen

Storage Conditions 培养条件Frozen 冷冻物 Cultures:-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried 冻干物 Cultures:

2-8°C

Live Cultures:

See Protocols section for handling information

Type Strain no

Medium 培养基 ATCC Medium 2154: LYI Entamoeba medium

ATCC Medium 361: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 6.0 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use

Growth Conditions 生长条件Temperature 培养温度 : 35°C

Atmosphere 需氧情况 : Anaerobic 

Culture System: Axenic; pH 6.0

Cryopreservation Harvest and Preservation

Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.

Adjust the concentration of cells to 2 x 106  to  2 x 107/mL in fresh medium.

While cells are centrifuging prepare a 10% (v) solution of sterile DMSO in fresh medium.

Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube.

Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium.

Invert several times to dissolve the DMSO.

Allow to warm to room temperature.

Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to  107 cells/mL and 5% (v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.

Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  

The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.

Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0.

Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154).

Name of Depositor 寄存人 R Samuels

Year of Origin 1964 

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