Z-VAD-FMK

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产品描述：

产品名称：Z-VAD-FMK

参考文献:

[1] Slee EA, et al. Biochem J, 1996, 315 (Pt 1), 21-24.

[2] Shimizu T, et al. Leukemia, 1997, 11(8), 1238-1244.

[3] Ilangovan R, et al. J Biol Chem, 2003, 278(33), 30993-30999.

[4] Cowburn AS, et al. Blood, 2005, 105(7), 2970-2972.

[5] Kim WJ, et al. Exp Eye Res, 2000, 71(3), 225-232.

CAS号: 187389-52-2

靶点: Pan-caspase

描述:

Z-VAD-FMK是一种细胞渗透性的，不可逆泛caspase抑制剂，在THP.1 和 Jurkat T细胞中阻断细胞凋亡的所有特性。

别名: Z-VAD(OMe)-FMK；Z-Val-Ala-Asp(OMe)-FMK

外观: white powder

In vitro(体外研究): Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 mM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay.

分子式: C₂₂H₃₀FN₃O₇

In vivo(体内研究): In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo.

分子量: 467.49

纯度: ＞98%

储存/保存方法: store at -20℃ for one year（Powder）； in DMSO or others solvent store at 2-4℃ for two weeks, at -20℃ for six months.

可溶性/溶解性: DMSO : ≥ 30 mg/mL (64.17 mM)

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