公告提醒:爱必信所有产品和服务仅用于科学研究,不用于临床应用及其他用途提供产品和服务(也不为任何个人提供产品和服务)! 产品描述: 产品名称:Z-VAD-FMK 参考文献: [1] Slee EA, et al. Biochem J, 1996, 315 (Pt 1), 21-24. [2] Shimizu T, et al. Leukemia, 1997, 11(8), 1238-1244. [3] Ilangovan R, et al. J Biol Chem, 2003, 278(33), 30993-30999. [4] Cowburn AS, et al. Blood, 2005, 105(7), 2970-2972. [5] Kim WJ, et al. Exp Eye Res, 2000, 71(3), 225-232. CAS号: 187389-52-2 靶点: Pan-caspase 描述: Z-VAD-FMK是一种细胞渗透性的,不可逆泛caspase抑制剂,在THP.1 和 Jurkat T细胞中阻断细胞凋亡的所有特性。 别名: Z-VAD(OMe)-FMK;Z-Val-Ala-Asp(OMe)-FMK 外观: white powder In vitro(体外研究): Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 mM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. 分子式: C22H30FN3O7 In vivo(体内研究): In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo. 分子量: 467.49 纯度: >98% 储存/保存方法: store at -20℃ for one year(Powder); in DMSO or others solvent store at 2-4℃ for two weeks, at -20℃ for six months. 可溶性/溶解性: DMSO : ≥ 30 mg/mL (64.17 mM) 产品信息订购:
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