Recombinant Mouse Noggin, 小鼠蛋白Noggin

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产品描述：

产品名称：Recombinant Mouse Noggin

参考文献: 1. Davis SWandCamper SA. 2007. Dev Biol, 305: 145-60.<br/>2. Zhu W, Kim J, Cheng C, et al. 2006. Bone, 39: 61-71.<br/>3. Oxley CD, Rashid R, Goudie DR, et al. 2008. Horm Res, 69: 221-6.<br/>4. Cooper GM, Usas A, Olshanski A, et al. 2009. Plast Reconstr Surg, 123: 94S-103S.<br/>5. Bayramov AV, Eroshkin FM, Martynova NY, et al. 2011. Development, 138: 5345-56.

氨基酸序列: MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

Accession: <a href="http://www.uniprot.org/uniprot/P97466" target="_blank">P97466</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/18121" target="_blank">18121</a>

种属: Murine

纯度: > 95 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rMuNoggin as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg in the presence of 5 ng/ml BMP-4.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration less than 0.25 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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