Recombinant Mouse FGF-8, 小鼠蛋白FGF-8

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产品描述：

产品名称：Recombinant Mouse FGF-8

参考文献: 1. Powers CJ, McLeskey SW, Wellstein A. 2000. Endocr Relat Cancer. 7:165-97.<br/>2. Mattila MM, Harkonen PL. 2007. Cytokine Growth Factor Rev. 18:257-66.<br/>3. Tanaka A, Miyamoto K, Minamino N, et al. 1992. Proc Natl Acad Sci U S A. 89:8928-32.<br/>4. Gemel J, Gorry M, Ehrlich GD, et al. 1996. Genomics. 35:253-7.<br/>5. Blunt AG, Lawshe A, Cunningham ML, et al. 1997. J Biol Chem. 272:3733-8.<br/>6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.<br/>8. Olsen SK, Li JY, Bromleigh C, et al. 2006. Genes Dev. 20:185-98.

氨基酸序列: QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR

Accession: <a href="http://www.uniprot.org/uniprot/P37237" target="_blank">P37237</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/14179" target="_blank">14179</a>

种属: Murine

纯度: > 97 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rMuFGF-8 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg in the presence of 10 μg/ml of heparin.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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