Recombinant Mouse IL-10, 小鼠蛋白IL-10

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产品描述：

产品名称：Recombinant Mouse IL-10

氨基酸序列: SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS

参考文献: 1. Kim JM, Brannan CI, Copeland NG, et al. 1992. J Immunol. 148:3618-23.<br/>2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11.<br/>3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79.<br/>4. Vieira P, de Waal-Malefyt R, Dang MN, et al. 1991. Proc Natl Acad Sci U S A. 88:1172-6.<br/>5. Hsu DH, de Waal Malefyt R, Fiorentino DF, et al. 1990. Science. 250:830-2.

Accession: <a href="http://www.uniprot.org/uniprot/P18893" target="_blank">P18893</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/16153" target="_blank">16153</a>

种属: Murine

纯度: > 97 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rMuIL-10 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

形态: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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