Recombinant Mouse IL-6, 小鼠蛋白IL-6

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产品描述：

产品名称：Recombinant Mouse IL-6

参考文献: 1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8.<br/>2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44.<br/>3. Ming JE, Cernetti C, Steinman RM, et al. 1989. J Mol Cell Immunol. 4:203-11; discussion 211-2.<br/>4. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2.<br/>5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.<br/>6. Van Snick J, Cayphas S, Szikora JP, et al. 1988. Eur J Immunol. 18:193-7.<br/>

氨基酸序列: MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

Accession: <a href="http://www.uniprot.org/uniprot/ P08505" target="_blank"> P08505</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/16193" target="_blank">16193</a>

纯度: > 97 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rMuIL-6 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of > 5 × 10⁷ IU/mg.

形态: Lyophilized from a 0.2 μm filtered solution in 30 mM Acetic Acid, pH 3.0, 150 mM NaCl, 5 % Trehalose, 0.02 % Tween-20.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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