Recombinant Mouse IL-1β, 小鼠蛋白IL-1β

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产品描述：

产品名称：Recombinant Mouse IL-1β

氨基酸序列: VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS

参考文献: 1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.<br/>2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.<br/>3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22.<br/>4. Gray, P.W., D. Glaister, E. Chen, et al. 1986. J Immunol, 137: 3644-8.

Accession: <a href="http://www.uniprot.org/uniprot/P10749" target="_blank">P10749</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/16176" target="_blank">16176</a>

种属: Murine

纯度: > 96 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of rMuIL-1β as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 10⁸ IU/mg.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

形态: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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