Recombinant Human FGF-19, 人蛋白FGF-19

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产品描述：

产品名称：Recombinant Human FGF-19

氨基酸序列: MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK

参考文献: 1. Ladher RK, Anakwe KU, Gurney AL, et al. 2000. Science. 290:1965-7.<br/>2. Tomlinson E, Fu L, John L, et al. 2002. Endocrinology. 143:1741-7.<br/>3. Smallwood PM, Munoz-Sanjuan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7.<br/>4. Fu L, John LM, Adams SH, et al. 2004. Endocrinology. 145:2594-603.<br/>5. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.<br/>6. Kurosu H, Kuro OM. 2009. Mol Cell Endocrinol. 299:72-8.<br/>7. Lin BC, Wang M, Blackmore C, et al. 2007. J Biol Chem. 282:27277-84.<br/>8. Kharitonenkov A, Dunbar JD, Bina HA, et al. 2008. J Cell Physiol. 215:1-7.

Accession: <a href="http://www.uniprot.org/uniprot/O95750" target="_blank">O95750</a>

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/9965" target="_blank">9965</a>

种属: Human

纯度: > 95 % by SDS-PAGE and HPLC analyses.

来源: Escherichia coli.

内毒素水平: Less than 1 EU/μg of Recombinant HumanFGF-19 as determined by LAL method.

活性: Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized Recombinant HumanFGF R4 at 5 μg/ml can bind Recombinant HumanFGF-19 with a linear range of 3-200 ng/ml.<br/>Assay #2: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 10³ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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