NMT历史上的今天丨在转基因拟南芥中过表达景天苜蓿Cu/Zn超氧化物歧化酶提高了对氧化应激的抗性

NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。

NMT历史上的今天

2017年06月13日，中国林科院亚热带林业研究所卓仁英用NMT发表了标题为Overexpressing the Sedum alfredii CuZn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis的研究成果。

期刊：Frontiers in Plant Science

主题：在转基因拟南芥中过表达景天苜蓿Cu/Zn超氧化物歧化酶提高了对氧化应激的抗性

标题：Overexpressing the Sedum alfredii CuZn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis

检测指标：Cd²⁺流速

作者：中国林科院亚热带林业研究所卓仁英、韩小娇、李真

英文摘要

Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)- scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized.

The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities.

Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (H₂O₂) and superoxide radicals (O₂ ). The influence of Cd stress on ion flux across the root surface showed that overexpressing SaCu/Zn SOD in transgenic Arabidopsis plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in Arabidopsis after Cd-induced oxidative stress, suggesting that SaCu/Zn SOD may participate in this network and enhance ROS-scavenging capability under Cd stress.

Taken together, these results suggest that overexpressing SaCu/Zn SOD increased oxidative stress resistance in transgenic Arabidopsis and provide useful information for understanding the role of SaCu/Zn SOD in response to abiotic stress.

中文摘要（谷歌机翻）

超氧化物歧化酶（SOD）是一种非常重要的活性氧（ROS）清除酶。在这项研究中，表征了景天科植物景天科植物镉（Cd）/锌/铅超积累的Cu / Zn SOD基因（SaCu / Zn SOD）的功能。

镉胁迫诱导了SaCu / Zn SOD的表达。与野生型植物相比，转基因拟南芥植物中SaCu / Zn SOD基因的过表达增强了其抗氧化防御能力，包括SOD和过氧化物酶活性。

此外，它还减少了与过氧化氢（H₂O₂）和超氧自由基（O₂）过量产生相关的损害。Cd胁迫对根表面离子通量的影响表明，过表达SaCu / Zn SOD在转基因拟南芥植物中具有更大的Cd吸收能力。基于微阵列数据的共表达网络表明，Cd诱导的氧化胁迫后拟南芥中可能存在氧化调控，这表明SaCu / Zn SOD可能参与该网络并增强Cd胁迫下的ROS清除能力。

综上所述，这些结果表明，过表达SaCu / Zn SOD在转基因拟南芥中提高了抗氧化胁迫的能力，并为理解SaCu / Zn SOD在响应非生物胁迫中的作用提供了有用的信息。

此外，暴露于Cd下的细胞壁厚度下降导致HE细胞壁中较少的细胞壁键合Cd。因此，Cd诱导的根部特征以及根尖的改变导致了两种生态型苜蓿链球菌对Cd吸收和积累的差异。

FIGURE 4 | Net Cd2C fluxes. Net Cd2C fluxes in the roots of transgenic (OE2, OE3, and OE4) and WT plants treated without (A) or with CdCl₂ stress (B), respectively. The average 300 s net Cd2C fluxes (C) are illustrated to highlight the trend differences. Bars indicate means  SD. Asterisks indicate significant differences at p < 0.05 and p < 0.01.