SCI REP-UK ：山东农大丨NaCl胁迫下磁处理可调控杨树N代谢

NMT是基因功能的活体检测技术，已被103位诺贝尔奖得主所在单位，及北大、清华、中科院使用。

期刊：Scientific Report

主题：NaCl胁迫下磁处理可调控杨树N代谢

标题：The effects of magnetic treatment on nitrogen   absorption and distribution in seedlings of Populus?×?euramericana   ‘Neva’ under NaCl stress

影响因子：4.011

检测指标：NH4+、NO3-流速

检测样品：杨树根伸长区（距根尖15mm）和叶肉细胞

NH4+、NO3-流实验处理方法：

4周龄杨树幼苗，分别经0g/L NaCl+磁化（M0），0g/L NaCl+非磁化（NM0），4g/L NaCl+磁化（M4），4g/L NaCl+非磁化（NM4）处理

NH4+、NO3-流实验测试液成份：
NH4+: 0.1 mM NH4NO3, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5
NO3-: 0.1 mM NH4NO3, 1.0 mM KCl, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5

作者：山东农业大学林学院马风云、刘秀梅

英文摘要

A potted experiment with Populus?×?euramericana   ‘Neva’ was carried out to assess whether there are positive effects of   magnetic treatment of saline water (MTSW) on nitrogen metabolism under   controlled conditions in a greenhouse. Growth properties, nitrogen   contents, enzyme activities and metabolite concentrations were   determined based on field experiments and laboratory analysis after a   30-day treatment.

The results were as follows: (1) Biomass  accumulation, root morphological properties and total nitrogen content  were improved by MTSW. (2) Magnetization led to a greater increase in  nitrate-nitrogen (NO3?-N) content in roots than in leaves, accompanied  by greater NO3? efflux and activated nitrate reductase. (3) MTSW led to a  higher ammonium-nitrogen (NH4+-N) content and greater uptake of net  NH4+ in the leaves than that in the roots. (4) Magnetization stimulated   glutamine synthase, glutamate dehydrogenase and glutamate synthase   activities, whereas the concentrations of glutathione and oxidized   glutathione were increased in leaves but decreased in roots, and the   total glutathione content was increased.

Overall, these results  indicated some beneficial impacts of MTSW on nitrogen translocation  under field conditions, especially for equilibrating the distribution of  NO3?-N and NH4+-N. Moreover, these findings confirmed the potential of  using low-quality water for agriculture.

中文摘要（谷歌机翻）

进行了盆栽的“ Neva”杨树的盆栽实验，以评估在受控条件下温室中磁化盐水（MTSW）对氮素代谢是否有积极作用。经过30天的处理后，根据田间实验和实验室分析确定了生长特性，氮含量，酶活性和代谢物浓度。

结果表明：（1）MTSW提高了生物量积累，根系形态特征和总氮含量。（2）磁化作用导致根部的**盐氮（NO3-N）含量比叶片中的增加更大，同时伴随着更大的NO3-流出量和活化的**还原酶。（3）MTSW导致叶片中的铵态氮（NH4+  -N）含量更高，并且比根部更高地吸收了净NH4   +。（4）磁化刺激谷氨酰胺合酶，谷氨酸脱氢酶和谷氨酸合酶活性，而叶片中谷胱甘肽和氧化型谷胱甘肽的浓度增加，而根部减少，谷胱甘肽总含量增加。

总体而言，这些结果表明，MTSW对田间条件下的氮转运有一些有益的影响，尤其是对于平衡NO3- -N和NH4+ -N的分布。此外，这些发现证实了将低质水用于农业的潜力。

结果表明：对于NO3-，叶肉细胞和根伸长区均表现为外排。存在NaCl胁迫时，磁疗前后，叶肉细胞的NO3-外排速率从235.09  pmol·cm-2·s-1（NM4）增加到290.23   pmol·cm-2·s-1（M4）；经NaCl胁迫后，根伸长区的NO3-外排速率从123.53   pmol·cm-2·s-1（M4）增加到157.51 pmol·cm-2·s-1（NM4）。

至于NH4+，叶肉细胞表现为吸收而根伸长区表现为外排。经NaCl胁迫后，叶肉细胞的NH4+吸收速率降低，NM0和NM4并无显著差异。但在根伸长区，NaCl胁迫后NH4+外排速率更高，M4的外排速率达到了186.83 pmol·cm-2·s-1。