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巢蛋白/神经上皮干细胞蛋白抗体
英文名称 Nestin
中文名称 巢蛋白/神经上皮干细胞蛋白抗体
别 名 ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.
研究领域 细胞生物 发育生物学 神经生物学 干细胞 细胞类型标志物
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Cow,
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 177kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Nestin:21-120/1621
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. 神经干细胞标志物
在动物体早期发育过程中,中枢神经系统发育尤为重要 。Nestin是一种中等纤维蛋白,它在哺乳动物神经前体细胞中表达较高,目前已被广泛用于神经前体细胞的重要标志分子。研究Nestin的表达调控规律,将有助于人们认识神经前体细胞的生物特性。此抗体还表达中枢神经系统干细胞及神经上皮细胞。