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磷酸化肝细胞生长因子受体(原癌基因)抗体
英文名称 Phospho-Met (Tyr1234 + Tyr1235)
中文名称 磷酸化肝细胞生长因子受体(原癌基因)抗体
别 名 Met(Phospho-Tyr1234); Met (c-Met) (phospho Y1234 + Y1235);c-Met (phospho Y1234 + Y1235); AUTS9; c met; cmet; D249; Hepatocyte growth factor receptor; Hepatocyte growth factor receptor Precursor; HGF; HGF receptor; HGF SF receptor; HGF/SF receptor; HGFR; MET; Met proto oncogene tyrosine kinase; Met proto-oncogene (hepatocyte growth factor receptor); Met proto-oncogene; Met protooncogene; MET_HUMAN; Oncogene MET; Par4; Proto-oncogene c-Met; RCCP2; Renal cell carcinoma papillary 2 gene; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met.
产品类型 磷酸化抗体
研究领域 肿瘤 染色质和核信号 信号转导 生长因子和激素 激酶和磷酸酶 表观遗传学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 153kDa
细胞定位 细胞膜 分泌型蛋白
性 状 Lyophilized or Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Met around the phosphorylation site of Tyr1234/1235:KE(p-Y)(p-Y)SV
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 c-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF). The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprising an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.
细胞膜受体(Membrane Receptors)
c-Met蛋白是肝细胞生长因子受体(Hepatocyte growth factor receptor, HGFR),又称受体蛋白酪氨酸激酶,肝细胞生长因子和过度表达的c-Met(HGFR)蛋白结合,在肿瘤的发生、进展和血管形成中都起着重要作用。
c-met蛋白也是HGF特异性受体,具有内源性酪氨酸激酶的活性,HGF与c-met蛋白特异性结合对肿瘤细胞生长、分化及恶性转化可能具有重要的关联。