The Hunk completely automates the chemical denaturation and protein stability measurement process. Easy set up in application-specific trays is all it takes to get started. Once the Hunk gets going, it’ll crank out up to 96 ΔGs on 96 different conditions fully unattended. With those ΔGs in hand, you’ll know right away whether or not it’s worth spending your time on minimizing aggregation or optimizing solubility and viscosity.
一小时即得ΔG
The Hunk’s got a much cooler way to get ΔG values. It adds increasing concentrations of denaturant to proteins until they completely unfold to get their denaturation curves. The midpoint of the curve, the C1/2, is the concentration of denaturant needed to onehalf unfold the protein. The Hunk multiplies that C1/2 by the slope (m value) of the curve at the C1/2 to get the ΔG for each of your proteins — and does it in about an hour.
预测蛋白聚集 With one Hunk experiment, you’ll know if and how your protein could aggregate. Follow ΔG over increasing protein concentrations, and get the ΔGtrend. When ΔGtrend goes up, you might want to check out a native state aggregation fix. When ΔGtrend goes down, you’ve got denatured state aggregation to deal with. A flat ΔGtrend means you’re good to go. In one day, the Hunk gives you more info than ever before about your protein’s aggregation pathway.