BTB/POZ结构域蛋白1elisa实验

BTB/POZ结构域蛋白1英文名称  Anti-BTBD1 

中文名称  BTB/POZ结构域蛋白1（丙型肝炎病毒的NS5A反转录蛋白8）抗体 

别    名  BTB (POZ) domain containing 1; BTB domain containing 1; BTB/POZ domain containing protein 1; BTBD 1; BTBD1; C15orf1; HCV NS5A transactivated protein 8; Hepatitis C virus NS5A transactivated protein 8; NS5ATP8; BTBD1_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg  

抗体来源  Rabbit  

BTB/POZ结构域蛋白1克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep   

产品类型  一抗    

研究领域  细胞生物 细胞周期蛋白 转录调节因子 细菌及病毒 细胞分化  

蛋白分子量  predicted molecular weight: 53kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human BTBD1 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 BTBD1 contains a proline-rich region and a BTB/POZ domain which are typically involved in protein-protein interactions. The C-terminus of BTBD1 binds topoisomerase I and BTBD1 also interacts with TRIM5 isoform Delta. BTBD1 localizes to cytoplasmic bodies and is essential for myogenic differentiation.

Function : Probable substrate-specific adapter of an E3 ubiquitin-protein ligase BTB/POZ结构域蛋白1complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 

Subunit : Interacts with topoisomerase 1 and with TRIM5 isoform Delta. Interacts with CUL3. 

Subcellular Location : Cytoplasm, P-body. Cytoplasmic bodies.

Tissue Specificity : Ubiquitous; higher levels in heart and skeletal muscle. 

Similarity : Contains 1 BTB (POZ) domain.

Database links : UniProtKB/Swiss-Prot: Q9H0C5.1