支链氨基酸转氨酶2抗体elisa实验

支链氨基酸转氨酶2抗体英文名称  Anti-BCAT2/BCAM 

中文名称  支链氨基酸转氨酶2抗体 

别    名  BCAM; BCAT 2; BCATM; BCT2; Branched chain aminotransferase 2, mitochondrial; ECA40; Placental protein 18; PP18. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg   

支链氨基酸转氨酶2抗体抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat  

产品类型  一抗    

研究领域  细胞生物 信号转导  

蛋白分子量  predicted molecular weight: 41kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human BCAT2/BCAM 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Class-IV pyridoxal-phosphate-dependent aminotransferase family members ECA39 and BCAT2 are both enzymes that catalyze the first reaction in the catabolism of the essential branched chain amino acids valine, leucine and isoleucine. ECA39, also known as BCAT1 (branched-chain-amino-acid aminotransferase 1, cytosolic) is localized to the cytoplasm where it forms a homodimer. ECA39 is expressed in the brain and kidney during embryogenesis and is overexpressed in c-Myc induced tumors. BCAT2 (branched-chain-amino-acid aminotransferase 2, mitochondrial), also known as placental protein 18 (PP18), is expressed as two isoforms produced by alternative splicing. The first isoform of BCAT2, designated BCAT2A, is expressed in the mitochondrion, while the second isoform, designated BCAT2B, is expressed in the cytoplasm. Ubiquitously expressed, BCAT2 is also thought to act as a transporter of branched chain α-keto acids. 

Function : Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

支链氨基酸转氨酶2抗体Subunit : Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Subcellular Location : Cytoplasm and Mitochondrion.

Tissue Specificity : Ubiquitous.

Similarity : Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. 

Database links : UniProtKB/Swiss-Prot: O15382.2