骨涎蛋白抗体elisa实验

骨涎蛋白抗体英文名称  Anti-Bone sialoprotein 

中文名称  骨涎蛋白抗体 

别    名  BNSP; Bone sialoprotein II; BSP; BSPII; Cell binding sialoprotein; Integrin binding sialoprotein; SPII; SIAL_HUMAN; Bone sialoprotein 2; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg  

抗体来源  Rabbit  

骨涎蛋白抗体克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat   

产品类型  一抗    

研究领域  生长因子和激素  

蛋白分子量  predicted molecular weight: 34kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human Bone sialoprotein II C-terminus 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12% of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

骨涎蛋白抗体Function : Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Subcellular Location : Secreted.

Post-translational modifications : N-glycosylated; glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains.

Sulfated on either Tyr-320 or Tyr-321 (By similarity).

Database links : UniProtKB/Swiss-Prot: P21815.4