TNF家族B细胞激活因子抗体elisa实验

TNF家族B细胞激活因子抗体英文名称  Anti-BAFF/CD257 

中文名称  TNF家族B细胞激活因子抗体 

别    名  ApoL related ligand TALL 1; B cell Activating Factor; B lymphocyte stimulator ; BLYS; CD 257; CD257; CD257 antigen; delta BAFF; Dendritic cell derived TNF like molecule; DTL; DTL precursor; TALL 1; TALL1; THANK; TNF and APOL related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF homolog that activates apoptosis NKFB and JNK; TNFSF13B; TNFSF20; Tumor necrosis factor ligand superfamily member 13B; Tumor necrosis factor ligand superfamily member 20; Tumor necrosis factor like protein ZTNF4; Tumor necrosis factor superfamily member 13B; ZTNF4; TN13B_MOUSE . 

浓    度  1mg/1ml 

TNF家族B细胞激活因子抗体规 格  0.2ml/200μg 

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Pig, Cow, Rabbit   

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 细胞凋亡 生长因子和激素 激酶和磷酸酶 细胞分化 b-淋巴细胞  

蛋白分子量  predicted molecular weight: 34kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from mouse BAFF C-terminus 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. [provided by RefSeq].

Function : Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

Subunit : Belongs to the tumor necrosis factor family.

Subcellular Location : Secreted and Cell membrane.

Tissue Specificity : Abundantly expressed in peripheral blood Leukocytes and is TNF家族B细胞激活因子抗体specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.

Post-translational modifications : The soluble form derives from the membrane form by proteolytic processing. 

Isoform 2 is not efficiently shed from the membrane unlike isoform 1.

Similarity : Belongs to the tumor necrosis factor family.

Database links : UniProtKB/Swiss-Prot: Q9WU72.1