Bcl-2同源结构域蛋白抗体elisa实验

Bcl-2同源结构域蛋白抗体英文名称  Anti-BECN1/Beclin 1/ATG6 

中文名称  Bcl-2同源结构域蛋白抗体 

别    名  Beclin 1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg  

抗体来源  Rabbit  

克隆类型  polyclonal 

交Bcl-2同源结构域蛋白抗体叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit   

产品类型  一抗    

研究领域  肿瘤 免疫学 神经生物学 细胞凋亡  

蛋白分子量  predicted molecular weight: 50kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human BECN1 (236-280aa) 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.

Function : Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Subunit : Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1.

Subcellular Location : Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.

Tissue Specificity : Ubiquitous.

Post-translational modifications : Phosphorylation at Thr-119 by DAPK1 reduces Bcl-2同源结构域蛋白抗体its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. 

Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.

Similarity : Belongs to the beclin family.

Database links : UniProtKB/Swiss-Prot: Q14457.2

BECN1是一种分子质量为60KDa的蛋白质, 它能与细胞凋亡抑制剂Bcl-2相互作用, 减少中枢神经系统的凋亡，参与PI3K复合物的组成和自噬体的形成。主要表达在胞质。抑癌基因beclin 1通过自噬作用来调节细胞生长。