Bcl2蛋白修饰因子抗体elisa实验

Bcl2蛋白修饰因子抗体英文名称  Anti-Bmf/Bcl2 modifying factor 

中文名称  Bcl2蛋白修饰因子抗体 

别    名  Bcl 2 modifying factor; Bcl-2-modifying factor; Bcl2 modifying factor; Bmf; BMF_HUMAN; FLJ00065. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg  

抗体来源  Rabbit  

Bcl2蛋白修饰因子抗体克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Cow, Horse, Rabbit   

产品类型  一抗    

研究领域  细胞生物 信号转导 细胞凋亡 细胞骨架  

蛋白分子量  predicted molecular weight: 20kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human Bcl2 modifying factor (54-88aa) 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The BH3-only proteins, which include Blk, Bad, Bik, Hrk, BID, Bim, NOXA, PUMA and Bmf, are proapoptotic members of the Bcl-2 family. Bcl-2 modifying factor (Bmf) is a BH3-only protein that binds prosurvival Bcl-2 family members to initiate apoptosis. Bmf is sequestered to Myosin V motors on Actin in the cytoskeleton by associating with Dynein light chain 2 (DLC2) homodimers. If the cell undergoes loss of attachment (anoikis), the cytoskeleton is disrupted and Bmf is released from DLC2. Bmf then translocates to the mitochondria, where Bcl-2 (an anti-apoptotic family member) is sequestered. The BH3 domain of Bmf facilitates binding to a hydrophobic groove on the surface of Bcl-2. Binding Bcl2蛋白修饰因子抗体results in a caspase cascade leading to apoptosis. Bmf is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues. The gene encoding Bmf maps to chromosome 15q14.

Function : May play a role in apoptosis. Isoform 1 seems to be the main initiator.

Subunit : Interacts with MCL1, BCL2, BCL2L1/BCL-Xl, BCL2A1 and BCL2L2/BCL-w. Interacts with the myosin V actin motor complex through its binding to DLC2

Tissue Specificity : Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.

Similarity : Belongs to the Bcl-2 family.