Na+/K+-ATPase α1 钠钾ATP酶α1抗体elisa实验

Na+/K+-ATPase α1 钠钾ATP酶α1抗体英文名称  Anti-ATP1A1 

中文名称  Na+/K+-ATPase α1 钠钾ATP酶α1抗体 

别    名  alpha 3 Sodium Potassium ATPase ; AT1A1_HUMAN; ATP1A1; Atpa-1; ATPase Na+/K+ transporting alpha 1 polypeptide; BC010319; EC 3.6.3.9; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase alpha 1 subunit; Na+/K+ ATPase 1; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; Sodium/potassium transporting ATPase alpha 1 chain; Sodium/potassium-transporting ATPase subunit alpha-1. 

浓    度  1mg/1ml 

Na+/K+-ATPase α1 钠钾ATP酶α1抗体规 格  0.2ml/200μg

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Rabbit   

产品类型  一抗    

研究领域  肿瘤 细胞生物 神经生物学 信号转导 通道蛋白 细胞膜受体 G蛋白偶联受体 新陈代谢 G蛋白信号  

蛋白分子量  predicted molecular weight: 112kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human ATP1A1 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as a oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport.The Alpha subunit contains the binding sites for ATP and the cations; the glycosylated Beta subunit ensures correct folding and membrane insertion of the Alpha subunits. The small subunit co-localizes with the Alpha subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The Betasubunit, but not the subunit, is essential for normal activity of Na+/K+-ATPase.

Function : This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Subunit : Interacts with SIK1. Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1. 

Na+/K+-ATPase α1 钠钾ATP酶α1抗体Subcellular Location : Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications : Phosphorylation on Tyr-10 modulates pumping activity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity

Similarity : Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.