磷酸化嗜中性粒细胞胞浆因子1抗体

英文名称  Anti-phospho-NCF1/p47 phox(Ser359) 

中文名称  磷酸化嗜中性粒细胞胞浆因子1抗体 

磷酸化嗜中性粒细胞胞浆因子1抗体别    名  phospho-NCF1(Ser359); phospho-p47 phox(Ser359); NCF1 (phospho S359); 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; NOXO2; p47 phox; p47phox; ;SH3 and PX domain containing protein 1A; SH3PXD1A; NADPH oxidase p47 phox; NCF1_HUMAN. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg   

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Rat   

产品类型  一抗  磷酸化抗体   

研究领域  肿瘤 细胞生物 信号转导  

蛋白分子量  predicted molecular weight: 45kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

磷酸化嗜中性粒细胞胞浆因子1抗体产品介绍 The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.

Function : NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Subunit : Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG. 

Subcellular Location : Cytoplasm.

Post-translational modifications : Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity. 

DISEASE : Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

Similarity : Contains 1 PX (phox homology) domain.

Contains 2 SH3 domains.

Database links : UniProtKB/Swiss-Prot: P14598.3