跨膜蛋白SEMA5A抗体

英文名称  Anti-SEMA5A/Semaphorin 5A 

中文名称  跨膜蛋白SEMA5A抗体 

跨膜蛋白SEMA5A抗体别    名  SEMA5A; SEMAF; Semaphorin F; SEM5A_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg   

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep 

产品类型  一抗    

研究领域  细胞生物 神经生物学 细胞膜蛋白  

蛋白分子量  predicted molecular weight: 118kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human SEMA5A/Semaphorin 5A 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

跨膜蛋白SEMA5A抗体产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.

Function : Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. 

跨膜蛋白SEMA5A抗体Subunit : Binds PLXNB3.

Subcellular Location : Membrane; single-pass type I membrane protein

Similarity : Belongs to the semaphorin family. 

Contains 1 PSI domain. 

Contains 1 Sema domain. 

Contains 7 TSP type-1 domains. 

Database links : UniProtKB/Swiss-Prot: Q13591.3