英文名称 Anti-SHP-1
中文名称 造血细胞磷酸酶抗体
造血细胞磷酸酶抗体别 名 SHP 1; SHP1; Src homology phosphatase-1; Src homology protein tyrosine phosphatase-1; hematopoietic cell phosphatase HCP; PTN6_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Cow
产品类型 一抗
研究领域 肿瘤 心血管 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 68kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SHP-1 C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
造血细胞磷酸酶抗体产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer. Alternative names: 70 kda SHP1L protein; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; PTP1C; PTPN6; SH PTP 1; SHP1; SHP1L.
Function : Modulates signaling by tyrosine phosphorylated cellcsurface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
造血细胞磷酸酶抗体Subunit : Monomer. Interacts with MTUS1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT (By similarity). Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1.
Subcellular Location : Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.
Tissue Specificity : Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Post-translational modifications : Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.
Similarity : Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
Database links : UniProtKB/Swiss-Prot: P29350.1
蛋白酪氨酸磷酸酶1(SHP-1)能是一个潜在的抑基因，它负性调节c-kit基因而抑制肿瘤的发生;
SHP-1为含SH2域(src homology-2 domain)的酪氨酸磷酸酶-1，主要表达于造血细胞，因此又称为造血细胞磷酸酶（hematopoietic cell phosphatase，HCP，也称为SHP-1或SHPTP1）。
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