突触相关蛋白25抗体elisa实验

英文名称  Anti-SNAP25 

中文名称  突触相关蛋白25抗体 

突触相关蛋白25抗体别    名  SNAP25; SNAP-25; bA416N4.2; Bdr; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SNAP; SNAP-25B; SNP 25; SNP25; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal Associated Protein 25; Synaptosomal associated protein 25kDa; SNP25_HUMAN . 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg 

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Cow, Rabbit 

产品类型  一抗    

研究领域  细胞生物 神经生物学 信号转导 生长因子和激素  

蛋白分子量  predicted molecular weight: 23kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human SNAP-25 C-terminus 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

突触相关蛋白25抗体保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Synaptosomal associated protein 25 kDa (snap-25) is a widely distributed membrane-associated protein in the brain, mainly localized in nerve terminals. 

Function : t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

Subunit : Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with CENPF, TRIM9, RIMS1, SNAPIN, OTOF and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1.

Subcellular Location : Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome.

Tissue Specificity : Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Post-translational modifications : Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.

Similarity : Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology d突触相关蛋白25抗体atabase links : UniProtKB/Swiss-Prot: P60880.1