细胞因子信号传导抑制蛋白1抗体elisa实验

英文名称  Anti-SOCS1 

中文名称  细胞因子信号传导抑制蛋白1抗体 

细胞因子信号传导抑制蛋白1抗体别    名  CISH 1; CISH1; Cytokine Inducible SH2 Protein 1; JAB; JAK Binding Protein; Janus kinase binding protein; SOCS 1; SOCS1; SOCS-1; SSI 1; SSI1; STAT Induced STAT Inhibitor 1; Supressor of cytokine Signalling 1; TEC Interacting Protein 3; TIP 3; TIP3. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg  1ml/1mg

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Pig, Cow, Horse, Rabbit

产品类型  一抗    

研究领域  细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子  

蛋白分子量  predicted molecular weight: 23kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human Socs 1 C-terminus 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 细胞因子信号传导抑制蛋白1抗体optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity (By similarity). Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor(LIF). Regulates interferon-gamma mediated sensory neuron survival. Implicated, through SOCS box binding, in ubiquitin-dependent protein degradation. High expression in thymus. Lower expression in lung and spleen.

Function : SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.

Subunit : Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV (By similarity). Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1 (By similarity). Interacts (via SH2 domain and SOCS box) with TRIM8 (By similarity). Interacts with AXL, CUL2 and FGFR3. Interacts with INSR (By similarity).

Subcellular Location : Nucleus. Cytoplasmic vesicle. Note=Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3.

Tissue Specificity : Expressed in all tissues with high expression in spleen, small 细胞因子信号传导抑制蛋白1抗体intestine and peripheral blood leukocytes.

Similarity : Contains 1 SH2 domain. Contains 1 SOCS box domain.

Database links : Entrez Gene: 8651 Human Entrez Gene: 12703 Mouse Entrez Gene: 252971 Rat Omim: 603597 Human SwissProt: O15524 Human SwissProt: O35716 Mouse SwissProt: Q9QX78 Rat Unigene: 50640 Human Unigene: 130 Mouse Unigene: 82754 Rat 

细胞因子信号传导抑制蛋白(suppressor of cytokine signaling, SOCS)家族是一类由细胞产生并反馈性阻断细胞因子信号转导过程的负性调节因子，SOCS1可抑制IL-6、LIF、OSM、INF-γ以及GH等多种细胞因子的信号转导，对体内多种免疫反应的激活起调控作用。SOCS1异常表达与多种疾病的发病相关，在急慢性白血病、类风湿性关节炎、硬化和的发病中起重要作用；对信号转导，特别是JAKSTAT通路的负调控作用已被肯定。