英文名称 Anti-Sin3b
中文名称 转录抑制蛋白Sin3b抗体
转录抑制蛋白Sin3b抗体别 名 SIN3 homolog B transcription regulator; Transcriptional corepressor Sin3b; SIN3B_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep
产品类型 一抗
研究领域 肿瘤 免疫学 染色质和核信号 转录调节因子
蛋白分子量 predicted molecular weight: 133kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Sin3b
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
转录抑制蛋白Sin3b抗体产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.
Function : Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription (By similarity).
Subunit : Interacts with FOXK1/MNF, MXI, MAD, NCOR1 and SAP30. Interaction with SUDS3 enhances the interaction with HDAC1 to form a complex. Interacts with MAD3, MAD4, MAEL, REST and SETDB1 (By similarity). Interacts with RNF220 (By similarity). Interacts with HCFC1.
Subcellular Location : Nucleus (By similarity).
Post-translational modifications : Ubiquitinated by RNF220 that leads to proteasomal degradation (By similarity).
转录抑制蛋白Sin3b抗体Similarity : Contains 3 PAH (paired amphipathic helix) domains.
Database links : UniProtKB/Swiss-Prot: O75182.2