小鼠糖原合成酶激酶-3β(GSK-3β)ELISA试剂盒
2015-09-11 18:32
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资料摘要:
Mouse GSK-3β
FOR RESEARCH USE ONLY
Assay range:6pmol/L - 200pmol/L 96 determinations
Purpose
This kit allows for the determination of GSK-3β concentrations in Mouse Hippocampal tissue and other biological fluids.
Principle of the assay
The kit assay Mouse GSK-3β level in the sample, use Purified Mouse GSK-3β antibody to coat microtiter plate wells, make solid-phase antibody, then add GSK-3β to wells, Combined GSK-3β antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse GSK-3β in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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小鼠糖原合成酶激酶-3β GSK-3β 英文说明书.doc
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