小鼠磷酸葡萄糖变位酶(PGM)ELISA试剂盒
2015-09-11 18:31
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资料摘要:
Mouse Phosphoglucomutase(PGM)
FOR RESEARCH USE ONLY
Assay range:1. 6U/L -60 U/L 96 determinations
Purpose
This kit allows for the determination of PGM concentrations in Mouse serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Mouse PGM level in the sample, use Purified Mouse PGM antibody to coat microtiter plate wells, make solid-phase antibody, then add PGM to wells, Combined PGM antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse PGM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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小鼠磷酸葡萄糖变位酶(PGM)英文说明书.doc
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