乙酸[AF法]检测试剂盒 K-ACETAF Megazyme现货产品促销

乙酸[AF法]检测试剂盒  K-ACETAF  Megazyme现货产品促销
产品货号：K-ACETAF 产品品名：乙酸[AF法]检测试剂盒 

订购热线： 400-811-9081

英文品名：ACETIC ACID(Acetyl-CoA Synthetase Format)assay Kit

规格型号：  (141.6 mL of reagent [R1 + R2] per kit;  equivalent to 456 reactions of 0.31 mL)

INTRODUCTION:
The most widely used method for enzymatic quantification of acetic acid is that based on the use of acetyl-coenzyme A synthetase (ACS), according to equations 1 – 3 below. For auto-analyser applications it becomes necessary to prepare a “master mix” reagent (R1), containing all components of the assay except the reaction initiation enzyme, ACS. However, reagent R1 when prepared from some kits has very limited on-machine stability, owing to a rapidly increasing absorbance value. To overcome this issue, Megazyme developed this kit (K-ACETAF), that doesn’t exhibit this instability phenomenon. Additionally, the anti-inhibitory compound polyvinylpyrollidone (PVP) has also been incorporated into the assay to prevent inhibition caused by certain tannins found in grape juice and wine.

KITS:
Kits suitable for the preparation of 141.6 mL of reagent (equivalent to 456 reactions of 0.31 mL) are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Buffer (30 mL, pH 8.4) plus L-malic acid, PVP and sodium azide (0.02 % w/v) as a preservative. Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+ plus ATP and CoA.Freeze dried powder.Stable for > 5 years at -20°C.
Bottle 3: L-Malate dehydrogenase plus citrate synthase suspension, 2.2 mL. Stable for > 2 years at 4°C.
Bottle 4: Acetyl-coenzyme A synthetase suspension (1.1 mL). Stable for > 2 years at 4°C.

REAGENT PREPARATION:
Preparation of R1:
Component Volume
bottle 1 (buffer) 5.50 mL
bottle 2 (NAD+/ATP/CoA) 2.20 mL (after adding 5.50 mL of H2O to bottle 2)
bottle 3 (L-MDH/CS) 0.44 mL (swirl to mix before use)
H2O 18.35 mL
Total volume 26.49 mL

Preparation of R2:
Component Volume
bottle 4 (ACS) 0.22 mL (swirl to mix before use)
H2O 1.60 mL
Total volume 1.82 mL

EXAMPLE METHOD:
R1: 0.290 mL
Sample: ~ 0.005 mL
R2: 0.020 mL
Reaction time: 15 min at either 20-25°C or 37°C
Wavelength: 340 nm
Prepared reagent stability: > 3 days when refrigerated
Calculation: endpoint
Reaction direction: increase
Linearity: up to 30 μg/mL of acetic acid in final reaction solution

乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49