直链支链淀粉检测试剂盒 Amylose/Amylopectin assay Kit K-AMYL Megazyme 现货促销

直链支链淀粉检测试剂盒 Amylose/Amylopectin assay Kit  K-AMYL   Megazyme   现货促销
产品货号：K-AMYL  品牌：Megazyme  

订购热线： 400-811-9081 产品品名：直链支链淀粉检测试剂盒

英文品名：Amylose/Amylopectin assay Kit 规格型号：100 assays per kit  

For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure.

INTRODUCTION:

Many of the properties of cereal starches that determine their suitability for particular end-uses are dependent upon their amylose/amylopectin ratios. These properties include gelatinisation and gelation characteristics, solubility, the formation of resistant starch, and, for rice, the cooking and textural characteristics of whole grains1-5. Thus, the measurement of the amylose content of starches is an important quality parameter for starch processing.
Amylose is most commonly determined in cereal starches by the potentiometric, ampometric or colourimetric measurement of the iodine binding capacity of the amylose with the resultant formation of amylose-iodine inclusion complexes6-10. However, these methods are subject to uncertainties. Amylopectin-iodine complexes also form, and these reduce the concentration of free iodine measured by the non-colourimetric methods and may absorb at similar wavelengths to amylose-iodine complexes in colourimetric methods. These complexes lead to an overestimation of the amylose, requiring corrections to be applied. Many of the other problems experienced in the use of these methods are detailed by Gibson et al.11.
The specific formation of amylopectin complexes with the lectin concanavalin A (Con A) offers an alternative approach to amylose measurement in starches, that is not subject to these uncertainties12,13. Under defined conditions of pH, temperature and ionic strength, Con A specifically complexes branched polysaccharides based on α-D-glucopyranosyl or α-D-mannopyranosyl units at multiple non-reducing end-groups with the formation of a precipitate. Thus, Con A effectively complexes the amylopectin component of starch but not the primarily linear amylose component.
The procedure described in this booklet13 is a modification of a Con A method developed by Yun and Matheson (1990)13. It uses an ethanol pretreatment step to remove lipids prior to analysis [modified from Morrison and Laignelet (1983)7].

KITS:

Kits suitable for performing 100 assays are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Freeze dried Con A.
Stable for > 5 years at -20°C.
Bottle 2: Amyloglucosidase [200 U on p-nitrophenyl
β-maltoside (i.e. 3300 U on starch at pH 4.5 at 40°C)] plus fungal α-amylase (500 U on Ceralpha Reagent at pH 5.0 and 40°C), 2 mL.
Stable for > 5 years at 4°C.
Bottle 3: GOPOD Reagent Buffer. Buffer (48 mL,
pH 7.4), p-hydroxybenzoic acid and sodium azide
(0.04% w/v).
Stable for > 4 years at 4°C.
Bottle 4: GOPOD Reagent Enzymes. Glucose oxidase plus peroxidase and 4-aminoantipyrine. Freeze- dried powder.
Stable for > 5 years at -20°C.
Bottle 5: D-Glucose standard solution (5 mL, 1.0 mg/mL) in 0.2% (w/v) benzoic acid.
Stable for > 5 years at room temperature.
Bottle 6: Starch reference sample (with specified content of amylose).
Stable for > 5 years at room temperature.

乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49