可吸收糖类和膳食纤维检测试剂盒 Available Carbohydrates/Dietary Fibre Assay Kit K-ACHDF Megazyme 现货促销

 可吸收糖类和膳食纤维检测试剂盒 Available Carbohydrates/Dietary Fibre Assay Kit K-ACHDF  Megazyme  现货促销
产品货号：K-ACHDF

品牌：Megazyme

订购热线： 400-811-9081

产品品名：可吸收糖类和膳食纤维检测试剂盒

英文品名：Available Carbohydrates/Dietary Fibre Assay Kit

规格型号：100 assays of each component

An integrated procedure for the measurement of available carbohydrates and dietary fibre in cereal products, fruit and vegetables and food products.

INTRODUCTION:
A need for information on the carbohydrate composition of foods for diabetics prompted McCance and Lawrence1 to attempt to measure this to gain results that would be of biological significance. They divided the carbohydrates in foods into two broad groups, ‘available’ and ‘unavailable’. The available carbohydrates, that is sugar plus starch, were defined as those that are digested and absorbed by the human small intestine and which are glucogenic. The unavailable carbohydrates were defined as those that are not digested by the endogenous secretions of the human digestive tract. These are now generally referred to as dietary fibre.
The concept of ‘available’ and ‘unavailable’ carbohydrates drew attention to the fact that some carbohydrates are not digested and absorbed in the small intestine but rather reach the large bowel where they are fermented. In the FAO/WHO report on “Carbohydrates in Human Nutrition”2, it was stated that these terms can be misleading since the so-called ‘unavailable’ carbohydrate can, in fact, provide energy to the body through fermentation. This committee recommended use of the term ‘glycemic’ (i.e. providing carbohydrate for metabolism) to replace ‘available’. However, this term has not gained widespread acceptance, possibly because there is some confusion with the well recognised term ‘glycemic index’. For this reason, we have decided to continue using the term ‘available carbohydrates’ for carbohydrates that are digested and absorbed by the human small intestine; these include D-glucose,
D-fructose, sucrose, maltodextrins, non-resistant starch and the
D-glucose component of lactose; all measured as D-glucose plus
D-fructose following enzymic hydrolysis.
Dietary fibre is a mixture of complex organic substances including a range of hydrophilic compounds such as soluble and insoluble polysaccharides and non-digestable oligosaccharides as well as non-swellable, more or less hydrophobic compounds such as cutins, suberins and lignins. The procedures for the determination of total dietary fibre as outlined in this booklet are based on the methods of Lee et al.3 and Prosky et al.4 (AOAC Official Method 991.43, AACC Method 32-07). These methods do not measure non-digestible oligosaccharides, as these remain soluble in the ethanolic solution used to precipitate high molecular weight dietary fibre components.

KITS:
Kits suitable for performing 100 assays of each are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (11 mL, pH 7.6) plus sodium azide(0.02% w/v) as a preservative.Stable for > 3 years at 4°C.
Bottle 2: NADP+ plus ATP.Stable for> 5 years at -20°C.
Bottle 3: Thermostable α-amylase (6 mL, 3,000 U/mL)(no. E-BLAAM).Stable for > 3 years at 4°C.
Bottle 4: Protease (10 mL, ~ 350 U/mL)(no. E-BSPRT).Stable for > 3 years at -20°C.
Bottle 5: Amyloglucosidase (20 mL, ~ 3,300 U/mL)(no. E-AMGDF).Stable for > 3 years at 4°C.
Bottle 6: Sucrase plus β-galactosidase, freeze-dried powder.Stable for > 3 years at -20°C.
Bottle 7: Hexokinase plus glucose-6-phosphate dehydrogenase suspension, 2.25 mL.Stable for> 3 years at 4°C.
Bottle 8: Phosphoglucose isomerase suspension (2.25 mL). Stable for > 3 years at 4°C.
Bottle 9: D-Glucose plus D-fructose standard solution(5 mL, 0.2 mg/mL of each sugar).Stable for > 3 years at room temperature.

EQUIPMENT (RECOMMENDED):
1. Duran? bottles (250 mL volume).
2. Fritted crucible, Corning No. 36060 Büchner, fritted disk, Pyrex
60 mL, pore size, coarse, ASTM 40-60 μm, or equivalent. Prepare as follows:
a. Ash overnight at 525°C in muffle furnace.
b. Remove Celite and ash material by using a vacuum.
c. Soak in 2% (v/v) Micro cleaning solution (reagent 6) at room temperature for 1 h.
d. Rinse crucibles with water and then deionised water.
e. For final rinse, use 15 mL acetone and air dry.
f. Add approximately 1.0 g Celite to dried crucibles and dry at 130°C to constant weight.
g. Cool crucible in desiccator for approximately 1 h and record weight of crucible containing Celite.
3. Filtering flask, heavy-walled, with 1-L side arm.
4. Rubber ring adaptors for use on filtering flasks.
5. Vacuum source: vacuum pump or aspirator with regulator capable of regulating vacuum.
6. Water bath, shaking, large-capacity (20-24 L) with covers; capable of maintaining temperature of 100°C; equipped with automatic timers for on-off operation.
7. Balance, 0.1 mg accuracy.
8. Ovens, two, mechanical convection, set at 103 ± 2°C and
130 ± 3°C.
9. Desiccator, airtight, with SiO2 or equivalent desiccant. Desiccant dried biweekly overnight in 130°C oven.
10. pH meter.
11. Pipettors and tips, 50-200 μL and 5 mL capacity.
12. Dispensers
a. 15 ± 0.5 mL for 78% (v/v) EtOH, 95% ethanol, and acetone.
b. 40 ± 0.5 mL for buffer.
13. Measuring cylinder, 500 mL.
14. Magnetic stirrers and stirring bars.
15. Rubber spatulas.
16. Muffle furnace, 525 ± 5°C.
17. Microfuge, capable of 12,000 g.

乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49