酵母葡聚糖酶检测试剂盒

酵母葡聚糖酶检测试剂盒Enzymatic Yeast Beta-Glucan Assay Kit K-EBHLG  Megazyme现货促销
产品货号：K-EBHLG   

品牌：Megazyme 产品品名：酵母葡聚糖酶检测试剂盒

英文品名：Enzymatic Yeast Beta-Glucan Assay Kit 规格型号：50 assays per kit

An enzymatic procedure for the measurement of 1,3:1,6-beta-glucan in yeast. Also measures 1,3-β-glucan.

INTRODUCTION:

(1-3)-β-Glucans are widely distributed in nature, especially in algae,
fungi and yeast, but also in higher plants. They serve a variety of
biological functions. They form the major structural components of
cell walls, they act as storage carbohydrates and they sometimes play
a protective role by forming at specific sites in response to particular
stimuli such as wounding.1 Yeast β-glucan substantially enhances the
function of the immune system by activating macrophages, one of the
primary defences of the immune system. Literature indicates that the
potent anti-tumour properties of polysaccharide fractions extracted
from certain strains of mushrooms, in particular Grifola frondosa
also known as Maitake, can be attributed to linear 6-branched
(1-3)-β-glucan.2-4 Similar properties have recently been assigned to
(1-3)(1-4)-β-glucans from cereal grains (e.g. oats and barley).
The presence in wine of a particular high molecular weight
(~ 800 Kd) β-glucan, produced by the fungus, Botrytis cinerea (grey
rot) leads to clarification and filtration problems.5 Scleroglucan
(from Sclerotium rofsii), an industrial thickening agent with remarkable
thickening properties, has a very similar structure.
Megazyme offers an acid hydrolysis/enzymic procedure (K-YBGL)
for the determination of β-glucan in yeast and mushroom. That
method is based on acid hydrolysis to measure total β-glucan and
specific enzymic determination of α-glucan. β-Glucan is determined
by difference. A totally enzymic procedure specific for β-glucan has
been published by Danielson et al.6 The method employs Lyticase
from Sigma-Aldrich and exo-1,3-β-glucosidase/β-glucanase from
Megazyme. This method works well, but its widespread adoption
is limited by the high cost of Lyticase. In this booklet we describe
an alternate, simple enzymic procedure for the measurement of
(1-3)(1-6)-β-glucan in yeast. The method also gives quantitation
measurement of β-glucan in curdlan, laminarin and cereal β-glucan
preparations. It does not give quantitative measurement of β-glucan
in mushrooms, the reasons for which are currently being researched.

KITS:

Kits suitable for carrying out 50 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: GlucazymeTM preparation (exo-1,3-β-glucanase,
endo-1,3-β-glucanase, β-glucosidase and chitinase
suspension), 2.2 mL.
Stable for > 4 years at 4°C.
Bottle 2: GOPOD Reagent Buffer. Buffer (48 mL,
pH 7.4), p-hydroxybenzoic acid and sodium azide
(0.4% w/v).
Stable for > 4 years at 4°C.
Bottle 3: GOPOD Reagent Enzymes. Glucose oxidase
plus peroxidase and 4-aminoantipyrine. Freeze
dried powder.
Stable for > 5 years at -20°C.
Bottle 4: D-Glucose standard solution (5 mL, 1.5 mg/mL)
in 0.2% w/v benzoic acid.
Stable for > 5 years at room temperature.
Bottle 5: Control yeast β-glucan preparation (~ 2 g,
β-glucan content stated on the bottle label).
Stable for > 5 years at room temperature.
Bottle 6: Control starch preparation (~ 2 g, 96%
starch dwb).
Stable for > 5 years at room temperature.

同时还常年提供以下产品试剂盒，大部分备有库存

乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49