内切纤维素酶检测试剂盒 endo-Cellulase Assay Kit K-CELLG3 Megazyme现货促销

内切纤维素酶检测试剂盒  endo-Cellulase Assay Kit K-CELLG3   Megazyme现货促销
产品货号：K-CELLG3  

订购热线： 400-811-9081

品牌：Megazyme

产品品名：内切纤维素酶检测试剂盒

英文品名：endo-Cellulase Assay Kit

规格型号：180/360

A soluble substrate for the specific measurement of cellulase (endo-1,4-β-glucanase) in enzyme preparations and fermentation products.

INTRODUCTION:

Cellulase (endo-1,4-β-glucanase) plays a key role in the hydrolysis
of cellulosic biomass to fermentable sugars. This enzyme also finds
widespread industrial applications in the modification of cellulosic
materials and in the degradation of mixed linkage 1,3;1,4-β-glucans.
Numerous methods are available for the measurement of cellulase,
including those based on increase in reducing sugar levels on
hydrolysis of CM-cellulose or 1,3:1,4-β-glucan1. endo-Cellulase
can be specifically assayed using viscometric methods with soluble
CM-cellulose 7M as substrate, or by employing soluble or insoluble
(crosslinked) dyed cellulose or mixed-linkage β-glucan. In general,
assays based on the use of dyed polysaccharides are standardised
against a reducing sugar method that employs either CM-cellulose or
β-glucan as substrate. The problem here is that these polysaccharide
substrates are not completely defined and thus lead to some variation
in the values obtained.
The Megazyme CellG3, cellulase test reagent, employs high purity
β-glucosidase and benzylidene blocked, 2-chloro-4-nitrophenyl-β-Dcellotrioside
(BClPNPβ-G3). The level of β-glucosidase used ensures
maximum sensitivity of the assay. On hydrolysis of BClPNPβ-G3 to
benzylidene blocked cellobiose and 2-Cl-4-nitrophenyl-β-D-glucose
by cellulase, the 2-Cl-4-nitrophenyl-β-D-glucose is immediately
cleaved to D-glucose and free 2-Cl-4-nitrophenol (ClPNP) by the
β-glucosidase present in the substrate mixture (Scheme 1). Thus, the
rate of release of ClPNP relates directly to the rate of hydrolysis of
BClPNPβ-G3 by cellulase. The reaction is stopped, and the phenolate
colour is developed, on addition of Trizma base solution (pH 9).
Standard curves relating enzyme activity to increase in absorbance
at 400 nm on hydrolysis of BClPNPβ-G3 by Trichoderma and A. niger
cellulases are shown in Figures 1 and 2. A standard curve relating
enzyme activity on CM-cellulose 4M (Nelson/Somogyi reducing
sugar assay2) to absorbance increase at 400 nm on incubation
of Trichoderma cellulase with BClPNPβ-G3 is shown in Figure 3.
Cellulase enzymes from different sources vary in their ability to
hydrolyse BClPNPβ-G3, so it is necessary to establish a specific
standard curve for each cellulase to allow accurate quantitation. The
assay can be used at temperatures up to 80°C and in the pH range of
4.5 to 8.0. With cellulase enzymes with activity at high pH values, it
is necessary to terminate the reaction with tri-sodium phosphate
(pH 11.0).
Of the cellulases evaluated in the current study, that with the lowest
rate of hydrolysis of BClPNPβ-G3 was the cellulase from A. niger
(E-CELAN, Megazyme). BClPNPβ-G3 substrate solution as supplied
in dimethyl sulphoxide (DMSO) is very stable when stored at 4°C or
1
-20°C. The substrate, CellG3 is stable for at least 7 days at 4°C and
for > 2 years at -20°C.

KITS:

Kits suitable for performing 180 / 360 assays are available from
Megazyme. The kits contain the full assay method plus:
Bottle 1: (x2) Benzylidene blocked, 2-chloro-4-nitrophenyl-β-Dcellotrioside
(BClPNPβ-G3; 3 mL) in DMSO.
Stable for > 2 years at -20°C.
Bottle 2: KCl solution (15 mL, 100 mM) plus
sodium azide (0.02% w/v).
Stable for approx. 4 years at 4°C.
Bottle 3: Thermostable β-glucosidase (0.40 mL, 400 U/mL) in
50% w/v ammonium sulphate solution plus sodium
azide (0.02% w/v).
Stable for approx. 4 years at 4°C.
Bottle 4: Trichoderma cellulase standard solution (5 mL,
~ 1.6 U/mL; actual value stated on the vial label) in
50% aqueous glycerol plus sodium azide (0.02% w/v).
Stable for approx. 4 years at -20°C.

同时还常年提供以下产品试剂盒，大部分备有库存

乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49