β-淀粉酶检测试剂盒Betamyl-3; β-amylase Assay Kit K-BETA3 Megazyme现货促销

β-淀粉酶检测试剂盒Betamyl-3; β-amylase Assay Kit  K-BETA3  Megazyme现货促销
产品货号：K-BETA3 

订购热线： 400-811-9081

品牌：Megazyme

产品品名：β-淀粉酶检测试剂盒

英文品名：Betamyl-3; β-amylase Assay Kit

规格型号： 100/200 assays per kit

For the specific measurement of beta-amylase in malt flour.

INTRODUCTION:

β-Amylase plays a central role in the complete degradation of starch to
metabolisable or fermentable sugars during the germination or malting
of cereal grains. It also finds considerable application, together with
starch debranching enzymes, in the production of high maltose syrups.
β-Amylase is usually measured using non-specific reducing sugar assays
with starch as substrate. In some methods, the α-amylase is first
inactivated by treatment at low pH.
A major advance in the assay of β-amylase was introduced by
Mathewson and Seabourn1 who found that the Calbiochem Pantrak?
serum α-amylase reagent could be used to measure β-amylase in the
presence of cereal α-amylase. The reagent (Pantrak) consists of a
mixture of p-nitrophenyl-α-D-maltopentaoside (PNPG5) and
p-nitrophenyl-α-D-maltohexaoside (PNPG6). These substrates are
rapidly hydrolysed by β-amylase, but are only slowly cleaved by cereal
α-amylase, which requires a longer stretch of α-1,4-linked D-glucosyl
residues to satisfy the substrate sub-site binding requirements.
Subsequently, Megazyme offered a product, Betamyl? (β-Amylase
Assay Reagent) that comprised just PNPG5 and α-glucosidase2,
which gave greater specificity. This reagent is now superceded by
the Megazyme Betamyl-3?, β-Amylase assay reagent, which is more
specific and considerably more stable than the Betamyl? reagent.
The Megazyme Betamyl-3?, β-amylase test reagent employs high
purity β-glucosidase and p-nitrophenyl-β-D-maltotrioside (PNPβ-G3).
The level of β-glucosidase used ensures maximum sensitivity of the
assay. On hydrolysis of PNPβ-G3 to maltose and p-nitrophenyl-β-Dglucose
by β-amylase, the p-nitrophenyl-β-D-glucose is immediately
cleaved to D-glucose and free p-nitrophenol by the β-glucosidase
present in the substrate mixture (Scheme 1). Thus, the rate of release
of p-nitrophenol relates directly to the rate of release of maltose
by β-amylase. The reaction is stopped, and the phenolate colour is
developed, on addition of a high pH Trizma base solution.
The PNPβ-G3 plus β-glucosidase mixture contains stabilisers which
significantly increase its stability. The blank absorbance value of the
Betamyl-3? substrate solution, when stored at 4 or 20°C, increases
much more slowly than similar increases for Betamyl?. reagent

KITS:

Kits suitable for performing 100 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: (x2) Each vial contains p-nitrophenyl-β-D-maltotrioside
(PNPβ-G3) plus β-glucosidase (50 U) and stabilisers.
Stable for > 4 years at -20°C.
Bottle 2: Tris/HCl buffer (25 mL, 1 M, pH 8.0) plus disodium
EDTA (20 mM) and sodium azide (0.02 % w/v).
Stable for approx. 4 years at 4°C.
Bottle 3: MES buffer (48 mL, 1 M, pH 6.2) plus disodium
EDTA (20 mM), BSA 10 mg/mL and sodium azide
(0.20 % w/v).
Stable for approx. 4 years at 4°C.
Bottle 4: Cysteine hydrochloride (16 g).
Stable for > 2 years at room temperature.
Bottle 5: Malt flour of standardised β-amylase activity (as
specified on bottle label).
Stable for > 4 years at room temperature.

同时还常年提供以下产品试剂盒，大部分备有库存

乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49