天冬酰胺、谷氨酰胺和氨快速检测试剂盒

天冬酰胺、谷氨酰胺和氨快速检测试剂盒L-Asparagine / L-Glutamine / Ammonia (Rapid) Assay Kit Megazyme现货促销
产品货号：K-ASNAM

品牌：Megazyme 

产品品名：天冬酰胺、谷氨酰胺和氨快速检测试剂盒

英文品名：L-Asparagine / L-Glutamine / Ammonia (Rapid) Assay Kit

规格型号：100 Assays (50 of each)

Novel method for the specific, convenient, cost effective and rapid assay of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media / supernatant components, or in other materials.

INTRODUCTION:

The simple and rapid measurement of L-asparagine and ammonia together has recently become of great significance with respect to two major applications:
1. Cell Culture: L-Asparagine is an essential component of certain cell culture media. However, the incorporation of this amino acid into growth media presents two major problems; firstly, L-asparagine is labile, and spontaneously breaks down to L-aspartate and free ammonium ions. Secondly, the released ammonium ions are very toxic to the cells. To overcome these issues L-asparagine is added just prior to use, and its concentration along with that of ammonia is frequently monitored during culturing.
This kit (K-ASNAM), based on the use of advanced recombinant enzymes, is very rapid (~ 20 min), and also measures both
L-asparagine and ammonia in a very simple format. A value for
L-glutamine is also produced as part of the method, that may also be of significance in cell culture applications. Both manual (see page 6, “A”) and microplate (see page 9, “B”) formats are described. Other rapid tissue culture test kits are also available from Megazyme for ammonia (K-AMIAR), L-glutamine / ammonia (K-GLNAM), D-glucose (K-GLUHKR or K-GLUC), and L-lactic acid (K-LATE).
2. Acrylamide Precursors: It is now well known that when
L-asparagine, ammonium ions, D-fructose and / or D-glucose are heated above approximately 160°C, significant levels of the carcinogenic compound acrylamide are formed, with obvious concerns for human health. Food products affected include potato crisps and chips, roasted potatoes and other fried, toasted or roasted foods, such as bakery goods, breakfast cereals and coffee. Asparaginase, an enzyme that converts L-asparagine into L-aspartic acid, thus has potential applications in this area for reducing acrylamide levels. However, if asparaginase treatment is adopted, it will be necessary to confirm that the free L-asparagine has been successfully converted to L-aspartic acid. This kit (K-ASNAM) is ideal for this application, and along with the Megazyme D-fructose / D-glucose kit (K-FRUGL) enables the concentrations of all four key acrylamide precursors to be determined both simply, and cost effectively.
This kit is also suitable for the analysis of L-asparagine, L-glutamine and ammonia in a wide range of other samples. Most notably, this method first converts / quantifies L-glutamine, an amino acid that would otherwise lead to interference due to the low but significant action of asparaginase on this compound.

KITS:

Kits suitable for performing 50 assays each of L-asparagine, L-glutamine and ammonia are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (11 mL, pH 4.9) plus sodium azide
(0.02% w/v) as
a preservative. Stable for > 2 years at 4°C.
Bottle 2: (x2) Buffer (25.5 mL, pH 8.0) plus 2-oxoglutarate and sodium azide (0.02% w/v) as a preservative.
Stable for
> 2 years at 4°C.
4
Bottle 3: (x2) NADPH. Lyophilised powder.
Stable for > 5 years at -20°C.
Bottle 4: Glutaminase suspension (1.1 mL).
Stable for
> 2 years at 4°C.
Bottle 5: Glutamate dehydrogenase suspension (2.2 mL). Stable for > 2 years at 4°C.
Bottle 6: Asparaginase suspension (1.1 mL).
Stable for
> 2 years at 4°C.
Bottle 7: Ammonia standard solution (5 mL, 0.04 mg/mL) in 0.02% sodium azide.
Stable for
> 2 years at 4°C.
Bottle 8: L-Asparagine control powder (~ 2 g).
Stable for
> 2 years at 4°C.杨树彦 

同时还常年提供以下产品试剂盒，大部分备有库存

乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49